A Phase III study in patients with chemo-refractory colorectal cancer with wild-type K-ras genotype demonstrated that Amgen’s Vectibix (panitumumab) improved overall survival compared to best supportive therapy, alone. A total of 377 patients were randomized to receive best supportive care with or without intravenous infusions of Vectibix every 14 days. Full results of the study will be presented at a future medical meeting and submitted for publication.
Vectibix is currently approved by the FDA in combination with FOLFOX for first-line treatment of patients with colorectal cancer, and as monotherapy following disease progression after prior treatment with fluoropyrimidine, oxaliplatin, and irinotecan-containing chemotherapy. Vectibix (panitumumab) is a recombinant, human IgG2 kappa monoclonal antibody that binds specifically to the human epidermal growth factor receptor (EGFR). Panitumumab has an approximate molecular weight of 147 kDa. Panitumumab is produced in genetically engineered mammalian (Chinese hamster ovary) cells.
What is Vectobix and how does it work?
The EGFR is a transmembrane glycoprotein that is a member of a subfamily of type I receptor tyrosine kinases, including EGFR, HER2, HER3, and HER4. EGFR is constitutively expressed in normal epithelial tissues, including the skin and hair follicle. EGFR is overexpressed in certain human cancers, including colon and rectum cancers. Interaction of EGFR with its normal ligands (eg, EGF, transforming growth factor-alpha) leads to phosphorylation and activation of a series of intracellular proteins, which in turn regulate transcription of genes involved with cellular growth and survival, motility, and proliferation. KRAS (Kirsten rat sarcoma 2 viral oncogene homologue) and NRAS (Neuroblastoma RAS viral oncogene homologue) are highly related members of the RAS oncogene family. Signal transduction through the EGFR can result in activation of the wild-type KRAS and NRAS proteins; however, in cells with activating RAS somatic mutations, the RAS-mutant proteins are continuously active and appear independent of EGFR regulation.
Panitumumab binds specifically to EGFR on both normal and tumor cells, and competitively inhibits the binding of ligands for EGFR. Nonclinical studies show that binding of panitumumab to the EGFR prevents ligand-induced receptor auto-phosphorylation and activation of receptor-associated kinases, resulting in inhibition of cell growth, induction of apoptosis, decreased pro-inflammatory cytokine and vascular growth factor production, and internalization of the EGFR. In vitro assays and in vivo animal studies demonstrate that panitumumab inhibits the growth and survival of selected human tumor cell lines expressing EGFR.
What is FOLFOX?
FOLFOX is a chemotherapeutic regimen consisting of oxaloplatin, 5-fluorouracil (5-FU) and leucovorin. Oxaliplatin, like cisplatin and carboplatin, acts by causing DNA adducts (covalently bound molecules) that disrupt replication, causing point mutations and DNA double strand breaks, which leads to apoptosis.
5-FU active metabolite, fluorodeoxyuridine monophosphate (FdUMP), disrupts thymidine triphosphate metabolism, which impedes DNA replication and transcription since deoxy-thymidine triphosphate is a requisite DNA nucleotide. Leucovorin potentiates 5-FU activity by increasing the levels of 5,10-methylene-tetrahydrofolate (CH2-THF ), which is critical for thymidylate synthase.